ATP12A Polyclonal antibody

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  WB analysis of HEK-293 using 13231-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13231-1-AP (ATP12A Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 13231-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13231-1-AP (ATP12A Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ATP12A Polyclonal Antibody for WB, ELISA

Cat No. 13231-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ATP12A, ATP1AL1, Proton pump

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13231-1-AP targets ATP12A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4105

  Product name: Recombinant human ATP12A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 367-700 aa of BC031609

  Sequence: LTAKRMAKKNCLVKNLEAVETLGSTSIICSDKTGTLTQNRMTVAHLWFDNQIFVADTSEDHSNQVFDQSSRTWASLSKIITLCNRAEFKPGQENVPIMKKAVIGDASETALLKFSEVILGDVMEIRKRNRKVAEIPFNSTNKFQLSIHEMDDPHGKRFLMVMKGAPERILEKCSTIMINGEEHPLDKSTAKTFHTAYMELGGLGERVLGFCHLYLPADEFPETYSFDIDAMNFPTSNLCFVGLLSMIDPPRSTVPDAVTKCRSAGIKVIMVTGDHPITAKAIAKSVGIISANSETVEDIAHRLNIAVEQVNKRDAKAAVVTGMELKDMSSEQLD

• Full Name: ATPase, H+/K+ transporting, nongastric, alpha polypeptide
• Calculated Molecular Weight: 1045 aa, 116 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC031609
• Gene Symbol: ATP12A
• Gene ID (NCBI): 479
• RRID: AB_2877926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P54707
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP12A, also named as ATP1AL1 and Proton pump, belongs to the family of P-type cation transport ATPases. It catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. ATP12A is responsible for potassium absorption in various tissues. Expression of ATP12A is increased in benign prostate hyperplasia and tumor tissues.