ATP13A1 Recombinant monoclonal antibody

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• ATP13A1 Recombinant monoclonal antibody (86762-3-RR)

• 

  WB analysis of HEK-293 using 86762-3-RR

  37°C incubated HEK-293 cells were subjected to SDS PAGE followed by western blot with 86762-3-RR (ATP13A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 86762-3-RR

  37°C incubated mouse liver tissue were subjected to SDS PAGE followed by western blot with 86762-3-RR (ATP13A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 86762-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86762-3-RR (ATP13A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 86762-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86762-3-RR (ATP13A1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 86762-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP13A1 antibody (86762-3-RR, Clone: 251765A3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86762-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86762-3-RR against Human ATP13A1 were performed. The affinity constant is 0.629 nM.

ATP13A1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 86762-3-RR

Clone No.251765A3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ATP13A, CGI-152, EC:7.4.2.-, Endoplasmic reticulum P5A-ATPase, Endoplasmic reticulum transmembrane helix translocase

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Tested Applications

• Positive WB detected in: 37°C incubated HEK-293 cells, 37°C incubated mouse liver tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86762-3-RR targets ATP13A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9174

  Product name: Recombinant human ATP13A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 615-758 aa of BC009302

  Sequence: QKEFVITSLKELGYVTLMCGDGTNDVGALKHADVGVALLANAPERVVERRRRPRDSPTLSNSGIRATSRTAKQRSGLPPSEEQPTSQRDRLSQVLRDLEDESTPIVKLGDASIAAPFTSKLSSIQCICHVIKQGRCTLVTTLQM

• Full Name: ATPase type 13A1
• Calculated Molecular Weight: 976 aa, 109 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC009302
• Gene Symbol: ATP13A1
• Gene ID (NCBI): 57130
• RRID: AB_3745107
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9HD20
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP13A1 is a P-type ATPase, mainly involved in the transport of cations within cells. It plays an important role in maintaining intracellular ion homeostasis and membrane potential. ATP13A1 acts as a dislocase that mediates the ATP-dependent extraction of mislocalized mitochondrial transmembrane proteins from the endoplasmic reticulum membrane (PubMed:32973005).