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WB analysis using 32764-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 32764-1-AP (ATP1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 32764-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 32764-1-AP (ATP1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 32764-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32764-1-AP (ATP1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 32764-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32764-1-AP (ATP1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 32764-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32764-1-AP (ATP1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 32764-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32764-1-AP (ATP1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATP1B1 Polyclonal antibody

Name: ATP1B1 Polyclonal antibody
Brand: Proteintech
SKU: 32764-1-AP

ATP1B1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32764-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ATP1B 1, Atp4b, Beta 1 subunit of Na(+) K(+) ATPase, Na, K-ATPase β1, Na+/K+ ATPase beta 1 subunit

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	fetal human brain tissue, mouse brain tissue, mouse liver tissue, rat brain tissue, rat heart tissue, mouse heart tissue
Positive IHC detected in	mouse liver tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below

Product Information

32764-1-AP targets ATP1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Eg3007 Product name: Recombinant Mouse ATP1B1 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 63-304 aa of NM_009721.6 Sequence: ELKPTYQDRVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLNIIRFLEKYKDSAQKDDMIFEDCGNVPSEPKERGDINHERGERKVCRFKLDWLGNCSGLNDDSYGYREGKPCIIIKLNRVLGFKPKPPKNESLETYPLMMKYNPNVLPVQCTGKRDEDKDKVGNIEYFGMGGYYGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTVDTEIRVECKAYGENIGYSEKDRFQGRFDVKIEIKS Predict reactive species
Full Name	ATPase, Na+/K+ transporting, beta 1 polypeptide
Calculated Molecular Weight	35 kDa
Observed Molecular Weight	40-50 kDa
GenBank Accession Number	NM_009721.6
Gene Symbol	Atp1b1
Gene ID (NCBI)	11931
RRID	AB_3742738
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity Purification
UNIPROT ID	P14094
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)