SERCA3 Polyclonal antibody

SERCA3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13619-1-AP

Synonyms

ATP2A3, Calcium pump 3, SERCA3, SR Ca(2+) ATPase 3



Tested Applications

Positive WB detected inmouse heart tissue, K-562 cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

13619-1-AP targets SERCA3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SERCA3 fusion protein Ag4527
Full Name ATPase, Ca++ transporting, ubiquitous
Calculated molecular weight 1043 aa, 114 kDa
Observed molecular weight 97-110 kDa
GenBank accession numberBC035729
Gene symbol ATP2A3
Gene ID (NCBI) 489
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The SERCA, which pumps cytosolic Ca2+ into the ER, is an imperative maintainer of ER Ca2+ homeostasis. The mammalian SERCA family is comprised of three tissue-specific members, SERCA1-3. SERCA3 is present in endothelial and epithelial cells of various tissues. SERCA3 helps regulate intracellular Ca2+, which is critical for maintaining normal INS secretion and is closely linked to metabolism, neuronal plasticity, gene transcription, cell growth, differentiation, apoptosis, protein folding and carcinogenesis. Several isoforms of SERCA3 exist due to the alternative splicing.

Protocols

Product Specific Protocols
WB protocol for SERCA3 antibody 13619-1-APDownload protocol
IHC protocol for SERCA3 antibody 13619-1-APDownload protocol
IP protocol for SERCA3 antibody 13619-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Pharmacol

Astragaloside IV Attenuates Podocyte Apoptosis Mediated by Endoplasmic Reticulum Stress through Upregulating Sarco/Endoplasmic Reticulum Ca(2+)-ATPase 2 Expression in Diabetic Nephropathy.

Authors - Hengjiang Guo