Tested Applications
| Positive WB detected in | mouse brain tissue, HeLa cells, human heart tissue, human skeletal muscle tissue, mouse skeletal muscle tissue, rat brain tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10910-1-AP targets ATP5C1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1343 Product name: Recombinant human ATP5C1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-298 aa of BC000931 Sequence: MFSRAGVAGLSAWTLQPQWIQVRNMATLKDITRRLKSIKNIQKITKSMKMVAAAKYARAERELKPARIYGLGSLALYEKADIKGPEDKKKHLLIGVSSDRGLCGAIHSSIAKQMKSEVATLTAAGKEVMLVGIGDKIRGILYRTHSDQFLVAFKEVGRKPPTFGDASVIALELLNSGYEFDEGSIIFNKFRSVISYKTEEKPIFSLNTVASADSMSIYDDIDADVLQNYQEYNLANIIYYSLKESTTSEQSARMTAMDNASKNASEMIDKLTLTFNRTRQAVITKELIEIISGAAALD Predict reactive species |
| Full Name | ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC000931 |
| Gene Symbol | ATP5C1 |
| Gene ID (NCBI) | 509 |
| RRID | AB_2877740 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P36542 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATP5C1(ATP synthase subunit gamma, mitochondrial) is also named as ATP5C, ATP5CL1 and belongs to the ATPase gamma chain family. Some scientists reported the complete sequence of the gene for the human ATP synthase gamma subunit and described tissue-specific isoforms of the subunit generated by alternative splicing of exon 9. The liver (L) isoform differed from the heart (H) isoform by the addition of a single amino acid (asp273) at the C terminus. (PMID:8227057).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP5C1 antibody 10910-1-AP | Download protocol |
| IHC protocol for ATP5C1 antibody 10910-1-AP | Download protocol |
| WB protocol for ATP5C1 antibody 10910-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATP5C1 Polyclonal antibody (catalog no. 10910-1-AP) has 15 citations. These appear in journals including Autophagy, Acta Pharm Sin B, Advanced Science, Science Advances, Journal of Experimental Medicine, Nature Structural & Molecular Biology, Apoptosis, International Journal of Biological Macromolecules, iScience, Journal of Biological Chemistry, Glycobiology, FEBS Letters, PLoS One, and bioRxiv. Research fields span mitochondrial biology, oxidative phosphorylation, cancer metabolism (Warburg effect), Parkinson's disease, ALS, acute kidney injury, cardiac hypertrophy, and neurodegeneration, with applications mainly in Western blot, immunofluorescence, and IHC.
| Species | Application | Title |
|---|---|---|
Autophagy AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells. | ||
Acta Pharm Sin B Cannabidiol alleviates methamphetamine addiction via targeting ATP5A1 and modulating the ATP-ADO-A1R signaling pathway. | ||
Adv Sci (Weinh) Icaritin Ameliorates Cisplatin-Induced Mitochondrial Metabolic Dysfunction-Associated Nephrotoxicity and Synergistically Potentiates Its Antitumor Efficacy. | ||
Adv Sci (Weinh) Single-Mitochondrion ATP Profiling Directs Discovery of Targetable OXPHOS Dependency in Cancers. | ||
J Exp Med Viral proteases activate the CARD8 inflammasome in the human cardiovascular system |
Reviews
What customers say
The single review for this product rates it 4 out of 5 stars. The reviewer used it at a 1:1000 dilution for Western blot on zebrafish tissue and reported positive results, confirming the antibody worked well in that application. Overall, the feedback is favorable, with the user expressing satisfaction with the performance on zebrafish WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lenie (Verified Customer) (12-18-2025) | Worked on zebrafish Western blot!
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