ATP6 Polyclonal antibody

ATP6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55313-1-AP

Synonyms

ATP synthase 6; ATPase subunit 6, ATP synthase subunit a, ATP6, ATPASE6, F ATPase protein 6, MT-ATP6



Tested Applications

Positive WB detected inmouse brain tissue, C2C12 cells, rat brain

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55313-1-AP targets ATP6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ATP synthase 6; ATPase subunit 6
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberJ01415
Gene Symbol ATP6
Gene ID (NCBI) 4508
RRIDAB_2881305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP synthase, also known as FoF1 complex, is a critical mitochondrial OXPHOS enzyme involved in the regulation of mitochondrial ATP production and in the maintenance of the mitochondrial membrane potential. It is composed of three components (F1, Fo and the peripheral stalk). F1, the soluble catalytic core, is above the membrane, inside the matrix of the mitochondria; consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon); Fo, comprising the proton channel, is within the membrane; Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP6 is the a subunit of Fo region.

Protocols

Product Specific Protocols
WB protocol for ATP6 antibody 55313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
humanWB

Nat Struct Mol Biol

Single-nucleoid architecture reveals heterogeneous packaging of mitochondrial DNA

Authors - R Stefan Isaac
humanWB

Mol Cell

Base-Resolution Mapping Reveals Distinct m1A Methylome in Nuclear- and Mitochondrial-Encoded Transcripts.

Authors - Xiaoyu Li
humanWB

Nat Commun

RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.

Authors - Uwe Richter
humanWB

Nucleic Acids Res

A coronary artery disease-associated tRNAThr mutation altered mitochondrial function, apoptosis and angiogenesis.

Authors - Zidong Jia
human, mouseWB

Nat Commun

Deregulation of mitochondrial F1FO-ATP synthase via OSCP in Alzheimer's disease.

Authors - Simon J Beck