ATP6V1B2 Monoclonal antibody, PBS Only

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  WB analysis using 68441-1-Ig (same clone as 68441-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68441-1-Ig (ATP6V1B2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68441-1-Ig (same clone as 68441-1-PBS)

  WB result of ATP6V1B2 antibody (68441-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1B2 transfected A549 cells. This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68441-1-Ig (same clone as 68441-1-PBS)

  IP result of anti-ATP6V1B2 (IP:68441-1-Ig, 4ug; Detection:68441-1-Ig 1:4000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68441-1-Ig (same clone as 68441-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug ATP6V1B2 Monoclonal antibody (68441-1-Ig, Clone:3C11A9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ATP6V1B2 Monoclonal Antibody for WB, FC (Intra), IP, Indirect ELISA

Cat No. 68441-1-PBS

Clone No.3C11A9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, FC (Intra), IP, Indirect ELISA

Synonyms

3C11A9, ATP6B1B2, ATP6B2, HO57, VATB

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Product Information

68441-1-PBS targets ATP6V1B2 in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7265

  Product name: Recombinant human ATP6V1B2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 162-511 aa of BC003100

  Sequence: CRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKH

• Full Name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 56-58 kDa
• GenBank Accession Number: BC003100
• Gene Symbol: ATP6V1B2
• Gene ID (NCBI): 526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P21281
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification(PMID:18667600).