CoraLite® Plus 488-conjugated ATP6V1B2 Recombinant monoclonal antibody

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• CoraLite® Plus 488-conjugated ATP6V1B2 Recombinant monoclonal antibody (CL488-87302-4)

ATP6V1B2 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-87302-4

Clone No.252572H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

ATP6B1B2, ATP6B2, HO57, VATB, V-ATPase subunit B 2

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  IF Staining of HeLa using CL488-87302-4

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ATP6V1B2 antibody (CL488-87302-4, Clone: 252572H5 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-87302-4 targets ATP6V1B2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7174

  Product name: Recombinant human ATP6V1B2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 162-511 aa of BC003100

  Sequence: CRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKH

• Full Name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 56-58 kDa
• GenBank Accession Number: BC003100
• Gene Symbol: ATP6V1B2
• Gene ID (NCBI): 526
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21281
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification (PMID:18667600). This antibody specifically recognizes ATP6V1B2 and does not cross-react with ATP6V1B1.