ATP6V1C1 Polyclonal antibody

ATP6V1C1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 16054-1-AP

Synonyms

ATP6C, ATP6D, ATP6V1C1, V ATPase subunit C 1, VATC, Vma5

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Validation Data Gallery

• 

  WB analysis using 16054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16054-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16054-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 16054-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 16054-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 16054-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, human brain tissue, human testis tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below

Product Information

16054-1-AP targets ATP6V1C1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP6V1C1 fusion protein Ag9020
• Full Name: ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1
• Calculated Molecular Weight: 382 aa, 44 kDa
• Observed Molecular Weight: 42-44 kDa
• GenBank Accession Number: BC010960
• Gene Symbol: ATP6V1C1
• Gene ID (NCBI): 528
• RRID: AB_2062501
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Ther; Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.; Authors - Benfu Zhong
mouse	WB	J Biol Chem; A brain-enriched Drp1 isoform associates with lysosomes, late endosomes and the plasma membrane.; Authors - Kie Itoh
human	WB	Commun Biol; A substrate-trapping strategy to find E3 ubiquitin ligase substrates identifies Parkin and TRIM28 targets.; Authors - Masashi Watanabe
human	WB	Biochem Biophys Res Commun; Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells.; Authors - Jong-Jer Lee
human	IHC	Bioengineered; A novel four-gene of iron metabolism-related and methylated for prognosis prediction of hepatocellular carcinoma.; Authors - Huimin Shen
human	WB	Autophagy; Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate; Authors - Mengting Ou