Tested Applications
| Positive WB detected in | U2OS cells, pig brain tissue, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68425-1-Ig targets ATP6V1H in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10130 Product name: Recombinant human ATP6V1H protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 136-483 aa of BC025275 Sequence: FTVHMAARIIAKLAAWGKELMEGSDLNYYFNWIKTQLSSQKLRGSGVAVETGTVSSSDSSQYVQCVAGCLQLMLRVNEYRFAWVEADGVNCIMGVLSNKCGFQLQYQMIFSIWLLAFSPQMCEHLRRYNIIPVLSDILQESVKEKVTRIILAAFRNFLEKSTERETRQEYALAMIQCKVLKQLENLEQQKYDDEDISEDIKFLLEKLGESVQDLSSFDEYSSELKSGRLEWSPVHKSEKFWRENAVRLNEKNYELLKILTKLLEVSDDPQVLAVAAHDVGEYVRHYPRGKRVIEQLGGKQLVMNHMHHEDQQVRYNALLAVQKLMVHNWEYLGKQLQSEQPQTAAARS Predict reactive species |
| Full Name | ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H |
| Calculated Molecular Weight | 483 aa, 56 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC025275 |
| Gene Symbol | ATP6V1H |
| Gene ID (NCBI) | 51606 |
| RRID | AB_2943428 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UI12 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP6V1H antibody 68425-1-Ig | Download protocol |
| WB protocol for ATP6V1H antibody 68425-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATP6V1H Monoclonal antibody (3G7B5), catalog number 68425-1-Ig, has 1 citation. It appears in Clinical Science (Clin Sci, Lond), a journal with an impact factor of 7.3. The cited study investigates steatotic hepatocyte-derived exosomes, free fatty acid uptake, miR-122-5p transport, and ATP6V1H-mediated CD36 translocation in hepatocytes, placing the research in the fields of liver disease (steatosis), exosome biology, and lipid metabolism.
| Species | Application | Title |
|---|---|---|
Clin Sci (Lond) Steatotic hepatocyte-derived exosomes increase free fatty acids uptake by transporting miR-122-5p to target ATP6V1H-mediated CD36 translocation in hepatocytes. |
Reviews
What customers say
The single review for this product rates it 5 stars. The customer used it for Western Blot at a 1:5000 dilution and described the performance as "very good for WB," indicating strong and reliable results in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ahmad (Verified Customer) (09-05-2026) | Very good for wb
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