ATP7A-Specific Polyclonal antibody, PBS Only

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• ATP7A-Specific Polyclonal antibody, PBS Only (55375-1-PBS)

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  WB analysis using 55375-1-AP (same clone as 55375-1-PBS)

  Unboiled mouse lung tissue were subjected to SDS PAGE followed by western blot with 55375-1-AP (ATP7A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55375-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse lung using 55375-1-AP (same clone as 55375-1-PBS)

  IP result of anti-ATP7A-Specific (IP:55375-1-AP, 4ug; Detection:55375-1-AP 1:500) with mouse lung tissue lysate 1520 ug. This data was developed using the same antibody clone with 55375-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 55375-1-AP (same clone as 55375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55375-1-AP (ATP7A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55375-1-PBS in a different storage buffer formulation.

ATP7A-Specific Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 55375-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

ATP7A, ATPase, Cu++ transporting, alpha polypeptide, Copper transporting ATPase 1

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Product Information

55375-1-PBS targets ATP7A-Specific in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: ATPase, Cu++ transporting, alpha polypeptide
• Calculated Molecular Weight: 165 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: NM_000052
• Gene Symbol: ATP7A
• Gene ID (NCBI): 538
• RRID: AB_3743207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q04656
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ATPase copper transporting alpha(ATP7A) is a type of transmembrane P-type ATPase for copper transport. ATP7A and ATP7B are homologous genes responsible for copper transport across cell membranes. ATP7A transports copper ions from the Golgi membrane to copper-dependent enzymes (such as dopamine β-hydroxylase) in the secretory pathway. When copper ion concentrations rise, ATP7A relocalizes from the Golgi apparatus to the plasma membrane, promoting copper efflux and thereby protecting cells from copper toxicity. Mutations in ATP7A are associated with Menkes disease (MNK)( PMID: 28389643, 30341172).