ATP7B-Specific Polyclonal antibody

ATP7B-Specific Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 19786-1-AP

Synonyms

ATP7B, Copper pump 2, Copper transporting ATPase 2, PWD, WC1, WD, WND



Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inmouse brain tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

19786-1-AP targets ATP7B-Specific in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ATPase, Cu++ transporting, beta polypeptide
Calculated molecular weight 157 kDa
Observed molecular weight 150 kDa
GenBank accession numberNM_000053
Gene symbol ATP7B
Gene ID (NCBI) 540
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP7B, also named as PWD, WC1 and WND, belongs to the cation transport ATPase (P-type) family and Type IB subfamily. ATP7B is involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. ATP7B catalyzes the reaction: ATP + H2O + Cu2+(In) = ADP + phosphate + Cu2+(Out). Defects in ATP7B are the cause of Wilson disease (WD). This antibody is specific to ATP7B.

Protocols

Product Specific Protocols
WB protocol for ATP7B-Specific antibody 19786-1-APDownload protocol
IHC protocol for ATP7B-Specific antibody 19786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Medicina (Kaunas)

Curcumin Effect on Copper Transport in HepG2 Cells.

Authors - Anita Berzina