Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IP detected in | Caco-2 cells |
| Positive IHC detected in | human colon cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19786-1-AP targets ATP7B-Specific in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ATPase, Cu++ transporting, beta polypeptide |
| Calculated Molecular Weight | 157 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | NM_000053 |
| Gene Symbol | ATP7B |
| Gene ID (NCBI) | 540 |
| RRID | AB_2878607 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35670 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATP7B, also named as PWD, WC1 and WND, belongs to the cation transport ATPase (P-type) family and Type IB subfamily. ATP7B is involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. ATP7B catalyzes the reaction: ATP + H2O + Cu2+(In) = ADP + phosphate + Cu2+(Out). Defects in ATP7B are the cause of Wilson disease (WD). This antibody is specific to ATP7B.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP7B-Specific antibody 19786-1-AP | Download protocol |
| IHC protocol for ATP7B-Specific antibody 19786-1-AP | Download protocol |
| IP protocol for ATP7B-Specific antibody 19786-1-AP | Download protocol |
| WB protocol for ATP7B-Specific antibody 19786-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ATP7B-Specific Polyclonal antibody (Cat# 19786-1-AP) has 35 citations across journals including J Adv Res, Adv Sci, Cell Reports Medicine, J Immunother Cancer, J Neuroinflammation, Acta Biomaterialia, Oncogenesis, Cell Signaling, Int Immunopharmacol, and iScience. Research fields span cuproptosis, copper homeostasis, cancer immunotherapy, hepatotoxicity, neuroinflammation, cardiotoxicity, ischemia/reperfusion injury, ferroptosis, and Wilson's disease.
| Species | Application | Title |
|---|---|---|
J Adv Res Cuproptosis in atrazine-induced hepatotoxicity: ATOX1 as a therapeutic target for lycopene-mediated protection | ||
Adv Sci (Weinh) Diallyl Trisulfide From Garlic Regulates RAB18 Phase Separation to Inhibit Lipophagy and Induce Cuproptosis in Hepatic Stellate Cells for Antifibrotic Effects | ||
Cell Rep Med Nilotinib induces immunogenic cuproptosis to potentiate cancer immunotherapy. | ||
J Immunother Cancer C5a/C5aR pathway blocking promoted CuS-mediated cancer therapy effect by inhibiting cuproptosis resistance | ||
J Neuroinflammation Aberrant copper metabolism and hepatic inflammation cause neurological manifestations in a mouse model of Wilson's disease | ||
Mater Today Bio Dual activation of cuproptosis and excessive autophagy by copper-bismuth metal-organic frameworks-loaded detachable microneedles in oral leukoplakia. |
