Tested Applications
| Positive WB detected in | HEK-293 cells, 143B cells, HepG2 cells, LNCaP cells, rat heart tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26723-1-AP targets ATP8 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, pig samples.
| Tested Reactivity | human, rat, pig |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ATP synthase 8; ATPase subunit 8 |
| Calculated Molecular Weight | 8 kDa |
| Observed Molecular Weight | 8 kDa |
| GenBank Accession Number | YP_003024030 |
| Gene Symbol | ATP8 |
| Gene ID (NCBI) | 4509 |
| RRID | AB_2880614 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P03928 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATP8 is a subunit of F0, the functional domain of mitochondrial ATP synthase or complex V. Mutation of ATP8 has been reported as the cause of apical hypertrophic cardiomyopathy and neuropathy.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP8 antibody 26723-1-AP | Download protocol |
| WB protocol for ATP8 antibody 26723-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATP8 Polyclonal antibody (Cat# 26723-1-AP) has 58 citations. Publications appear in high-impact journals including Cell, Nature Cell Biology, Nature Communications, Molecular Cell, EMBO Journal, Nucleic Acids Research, Journal of Biological Chemistry, Science Advances, Cell Metabolism, Redox Biology, and eLife, among others. Research fields span mitochondrial translation, tRNA metabolism, mitochondrial DNA mutations, cancer biology, cardiovascular disease, neurology, and hepatology, with most studies employing Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease | ||
Cell Dynamic mapping of proteome trafficking within and between living cells by TransitID | ||
Cell MTFP1 controls mitochondrial fusion to regulate inner membrane quality control and maintain mtDNA levels | ||
Cell Metab Aging-induced tRNAGlu-derived fragment impairs glutamate biosynthesis by targeting mitochondrial translation-dependent cristae organization | ||
Nat Cell Biol ALKBH7-mediated demethylation regulates mitochondrial polycistronic RNA processing. | ||
Nat Commun Mitochondrial translation termination, recycling, reinitiation, and rescue for in-frame and out-of-frame contexts. |
Reviews
What customers say
All four reviews are 5-star ratings for Western Blot. Users report sharp, well-defined single bands at dilutions ranging from 1:1000 to 1:3000 across fibroblasts, lymphoblastoid cells, and adipocytes. One reviewer noted the target migrates at a very low molecular weight (~8 kDa) and could be easily missed on the gel. Another confirmed a strong single band around 8 kD when loading 10 µg total protein in human adipocytes. Overall, the antibody is described as working perfectly with clear, reliable detection.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Emilie (Verified Customer) (09-24-2025) | Bands appeared sharp and well defined under the chosen dilution.
|
Manon (Verified Customer) (09-19-2025) | A distinct band is visible, but since it is at a very low molecular weight, it could be easily missed
|
Baptiste (Verified Customer) (05-23-2025) | Working perfectly
 |
Mi (Verified Customer) (02-10-2023) | This antibody works very well in human adipocytes, a strong single band around 8 kd is detected when loading 10 ug total protein.
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