Tested Applications
| Positive WB detected in | HEK-293 cells, 143B cells, HepG2 cells, LNCaP cells, rat heart tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 44 publications below |
Product Information
26723-1-AP targets ATP8 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, pig samples.
| Tested Reactivity | human, rat, pig |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
fusion protein Predict reactive species |
| Full Name | ATP synthase 8; ATPase subunit 8 |
| Calculated Molecular Weight | 8 kDa |
| Observed Molecular Weight | 8 kDa |
| GenBank Accession Number | YP_003024030 |
| Gene Symbol | ATP8 |
| Gene ID (NCBI) | 4509 |
| RRID | AB_2880614 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P03928 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATP8 is a subunit of F0, the functional domain of mitochondrial ATP synthase or complex V. Mutation of ATP8 has been reported as the cause of apical hypertrophic cardiomyopathy and neuropathy.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP8 antibody 26723-1-AP | Download protocol |
| IF protocol for ATP8 antibody 26723-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol ALKBH7-mediated demethylation regulates mitochondrial polycistronic RNA processing. | ||
Mol Cell Hepatic micropeptide modulates mitochondrial RNA processing machinery in hepatocellular carcinoma | ||
Nucleic Acids Res Selection of initiator tRNA and start codon by mammalian mitochondrial initiation factor 3 in leaderless mRNA translation | ||
Nucleic Acids Res A coronary artery disease-associated tRNAThr mutation altered mitochondrial function, apoptosis and angiogenesis. | ||
Nucleic Acids Res A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Emilie (Verified Customer) (09-24-2025) | Bands appeared sharp and well defined under the chosen dilution.
|
FH Manon (Verified Customer) (09-19-2025) | A distinct band is visible, but since it is at a very low molecular weight, it could be easily missed
|
FH Baptiste (Verified Customer) (05-23-2025) | Working perfectly
 |
FH Mi (Verified Customer) (02-10-2023) | This antibody works very well in human adipocytes, a strong single band around 8 kd is detected when loading 10 ug total protein.
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