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ATP8 Polyclonal antibody
ATP8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 26723-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, 143B cells, HepG2 cells, LNCaP cells, rat heart tissue |
Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 36 publications below |
Product Information
26723-1-AP targets ATP8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | ATP synthase 8; ATPase subunit 8 |
Calculated Molecular Weight | 8 kDa |
Observed Molecular Weight | 8 kDa |
GenBank Accession Number | YP_003024030 |
Gene Symbol | ATP8 |
Gene ID (NCBI) | 4509 |
RRID | AB_2880614 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATP8 is a subunit of F0, the functional domain of mitochondrial ATP synthase or complex V. Mutation of ATP8 has been reported as the cause of apical hypertrophic cardiomyopathy and neuropathy.
Protocols
Product Specific Protocols | |
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WB protocol for ATP8 antibody 26723-1-AP | Download protocol |
IHC protocol for ATP8 antibody 26723-1-AP | Download protocol |
IF protocol for ATP8 antibody 26723-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Cell Biol ALKBH7-mediated demethylation regulates mitochondrial polycistronic RNA processing. | ||
Nucleic Acids Res A coronary artery disease-associated tRNAThr mutation altered mitochondrial function, apoptosis and angiogenesis. | ||
Nucleic Acids Res A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation. | ||
Nucleic Acids Res Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency. | ||
Nucleic Acids Res Hypertension-associated mitochondrial DNA 4401A>G mutation caused the aberrant processing of tRNAMet, all 8 tRNAs and ND6 mRNA in the light-strand transcript. | ||
J Cell Biol Parkin-independent mitophagy via Drp1-mediated outer membrane severing and inner membrane ubiquitination. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Mi (Verified Customer) (02-10-2023) | This antibody works very well in human adipocytes, a strong single band around 8 kd is detected when loading 10 ug total protein.
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