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ATPB Polyclonal antibody

ATPB Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 17247-1-AP


ATP5B, ATPB, ATPMB, ATPSB, Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HT-29 cells, HepG2 cells, mouse brain tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17247-1-AP targets ATPB in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATPB fusion protein Ag11177
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Calculated Molecular Weight 529 aa, 57 kDa
Observed Molecular Weight45-57 kDa
GenBank Accession NumberBC016512
Gene Symbol ATP5B
Gene ID (NCBI) 506
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.


Product Specific Protocols
WB protocol for ATPB antibody 17247-1-APDownload protocol
IHC protocol for ATPB antibody 17247-1-APDownload protocol
IF protocol for ATPB antibody 17247-1-APDownload protocol
IP protocol for ATPB antibody 17247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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