Validation Data Gallery
|Positive WB detected in||HEK-293T cells, HeLa cells, mouse liver tissue, human heart tissue, rat heart tissue, rat liver tissue, HT-29 cells, HepG2 cells, mouse brain tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17247-1-AP targets ATPB in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATPB fusion protein Ag11177|
|Full Name||ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide|
|Calculated molecular weight||529 aa, 57 kDa|
|Observed molecular weight||45-57 kDa|
|GenBank accession number||BC016512|
|Gene ID (NCBI)||506|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.
|Product Specific Protocols|
|WB protocol for ATPB antibody 17247-1-AP||Download protocol|
|IHC protocol for ATPB antibody 17247-1-AP||Download protocol|
|IF protocol for ATPB antibody 17247-1-AP||Download protocol|
|IP protocol for ATPB antibody 17247-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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