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ATPB Polyclonal antibody

ATPB Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17247-1-AP

Synonyms

ATP5B, ATPB, ATPMB, ATPSB, Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker



Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, mouse liver tissue, human heart tissue, rat heart tissue, rat liver tissue, HT-29 cells, HepG2 cells, mouse brain tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17247-1-AP targets ATPB in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATPB fusion protein Ag11177
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Calculated molecular weight 529 aa, 57 kDa
Observed molecular weight45-57 kDa
GenBank accession numberBC016512
Gene symbol ATP5B
Gene ID (NCBI) 506
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.

Protocols

Product Specific Protocols
WB protocol for ATPB antibody 17247-1-APDownload protocol
IHC protocol for ATPB antibody 17247-1-APDownload protocol
IF protocol for ATPB antibody 17247-1-APDownload protocol
IP protocol for ATPB antibody 17247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.

Authors - Linbin Yang
humanWB

Nat Commun

Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease.

Authors - Di Hu
mouseWB

Nat Commun

ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models.

Authors - Yuanyuan Zhao
humanWB

Brain

Ligand-free mitochondria-localized mutant AR-induced cytotoxicity in spinal bulbar muscular atrophy.

Authors - Xia Feng
humanWB

Cell Death Differ

STING directly activates autophagy to tune the innate immune response.

Authors - Dong Liu
mouseWB

J Cell Biol

Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.

Authors - Uwe Richter