CoraLite®594-conjugated AURKA Monoclonal antibody
AURKA Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG3
Reactivity
Human, Mouse, Rat
Applications
IF
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1F4B10
Cat no : CL594-66757
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-66757 targets AURKA in IF applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Mouse / IgG3 |
Class | Monoclonal |
Type | Antibody |
Immunogen | AURKA fusion protein Ag20096 |
Full Name | aurora kinase A |
Calculated Molecular Weight | 46 kDa |
Observed Molecular Weight | 46-52 kDa |
GenBank Accession Number | BC002499 |
Gene Symbol | AURKA |
Gene ID (NCBI) | 6790 |
RRID | AB_2883624 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).
Protocols
Product Specific Protocols | |
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IF protocol for CL594 AURKA antibody CL594-66757 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |