Acetyl-Histone H3 (Lys9) Recombinant monoclonal antibody

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  WB analysis of HeLa using 84543-1-RR

  Trichostatin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control.

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  WB analysis of HEK-293 using 84543-1-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84543-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84543-1-RR, Clone: 241596F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Dot Blot experiment of peptide using 84543-1-RR

  Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

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  ChIP experiment of HeLa using 84543-1-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys9) (84543-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

Acetyl-Histone H3 (Lys9) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Cat No. 84543-1-RR

Clone No.241596F9

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Synonyms

Acetyl Histone, H3, H3 Ac Lys9, H3 Acetyl K9, H3 Acetyl Lys9

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Tested Applications

• Positive WB detected in: Trichostatin A treated HeLa cells, HEK-293 cells
• Positive IF/ICC detected in: HeLa cells
• Positive Dot Blot detected in: peptide
• Positive ChIP-qPCR detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• DOT BLOT: DOT BLOT : 1:10-1:100
• CHIP-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• ChIP: See 2 publications below

Product Information

84543-1-RR targets Acetyl-Histone H3 (Lys9) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• RRID: AB_3672048
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P68431
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	ChIP	Bone; Glucocorticoid receptor represses the LGR4 gene by binding to the nGRE sequence and recruiting HDAC4 in mice monocytes.; Authors - Qihang Fang
human	ChIP	BMC Cancer; Epigenetic activation of VEGFA by SMARCD1 mediates tumor progression and bevacizumab resistance in clear cell renal cell carcinoma.; Authors - Hanqing Liu
mouse	WB	Arch Gerontol Geriatr; A novel HDAC1-specific inhibitor prevents estrogen deficiency-induced osteoporosis in mice by inhibiting osteoclast function.; Authors - Hang Yuan
human	WB	Cell Mol Gastroenterol Hepatol; Gut Microbiota-Derived Propionate Governs Hepatic N2 Neutrophils in Wilson's Disease.; Authors - Xiaoxiao Mi
human	WB	Chem Biol Interact; The novel selective HDAC1 inhibitor ZJH-1 exhibits potent antitumor activity in castration-resistant prostate cancer, potentially involving HSP90AA1.; Authors - Yizhong Bao