Acetyl-Histone H4 (Lys91) Recombinant monoclonal antibody, PBS Only

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• Acetyl-Histone H4 (Lys91) Recombinant monoclonal antibody, PBS Only (86768-1-PBS)

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  WB analysis of NIH/3T3 using 86768-1-RR (same clone as 86768-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86768-1-RR (Acetyl-Histone H4 (Lys91) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86768-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 86768-1-RR (same clone as 86768-1-PBS)

  Trichostatin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 86768-1-RR (Acetyl-Histone H4 (Lys91) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86768-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86768-1-RR (same clone as 86768-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H4 (Lys91) antibody (86768-1-RR, Clone: 251157D9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86768-1-PBS in a different storage buffer formulation.

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  Dot Blot experiment of / using 86768-1-RR (same clone as 86768-1-PBS)

  Dot blot analysis was used to confirm the specificity of 86768-1-RR Acetyl-Histone H4 (Lys91) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 86768-1-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 86768-1-RR (same clone as 86768-1-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H4 (Lys91) (86768-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 86768-1-PBS in a different storage buffer formulation.

Acetyl-Histone H4 (Lys91) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Cat No. 86768-1-PBS

Clone No.251157D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Synonyms

Ac Histone H4, Ac Histone H4 (Lys91), Ac Histone H4 Lys91, Ac Histone H4-Lys91, Acetyl Histone

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Product Information

86768-1-PBS targets Acetyl-Histone H4 (Lys91) in WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H4a
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC069654
• Gene Symbol: HIST1H4A
• Gene ID (NCBI): 8359
• RRID: AB_3745109
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62805
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.