The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Various lysates were subjected to SDS PAGE followed by western blot with 86768-1-RR (Acetyl-Histone H4 (Lys91) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Various lysates were subjected to SDS PAGE followed by western blot with 86768-1-RR (Acetyl-Histone H4 (Lys91) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HSC-T6 using 86768-1-RR
Trichostatin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 86768-1-RR (Acetyl-Histone H4 (Lys91) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Trichostatin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 86768-1-RR (Acetyl-Histone H4 (Lys91) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 86768-1-RR
Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H4 (Lys91) antibody (86768-1-RR, Clone: 251157D9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H4 (Lys91) antibody (86768-1-RR, Clone: 251157D9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC experiment of NIH/3T3 using 86768-1-RR
1x10^6 Trichostatin A treated NIH/3T3 cells were intracellularly stained with 0.13 ug Acetyl-Histone H4 (Lys91) Recombinant monoclonal antibody (86768-1-RR, Clone:251157D9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
1x10^6 Trichostatin A treated NIH/3T3 cells were intracellularly stained with 0.13 ug Acetyl-Histone H4 (Lys91) Recombinant monoclonal antibody (86768-1-RR, Clone:251157D9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).
Dot Blot experiment of / using 86768-1-RR
Dot blot analysis was used to confirm the specificity of 86768-1-RR Acetyl-Histone H4 (Lys91) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Dot blot analysis was used to confirm the specificity of 86768-1-RR Acetyl-Histone H4 (Lys91) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.
ChIP experiment of HeLa using 86768-1-RR
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H4 (Lys91) (86768-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H4 (Lys91) (86768-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.
Trichostatin A treated NIH/3T3 cells, Trichostatin A treated HSC-T6 cells
Positive IF/ICC detected in
Trichostatin A treated HeLa cells
Positive FC (Intra) detected in
Trichostatin A treated NIH/3T3 cells
Positive Dot Blot detected in
/ /
Positive ChIP-qPCR detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC
IF/ICC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
DOT BLOT
DOT BLOT : 1:10-1:100
CHIP-QPCR
CHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
86768-1-RR targets Acetyl-Histone H4 (Lys91) in WB, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.
Protocols
Product Specific Protocols
FC protocol for Acetyl-Histone H4 (Lys91) antibody 86768-1-RR
This validation data has been generated in-house and reviewed by Proteintech scientists.
Find out more on our antibody validation policy
WB Figures
WB analysis of NIH/3T3 using 86768-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 86768-1-RR (Acetyl-Histone H4 (Lys91) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HSC-T6 using 86768-1-RR
Trichostatin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 86768-1-RR (Acetyl-Histone H4 (Lys91) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IF/ICC Figures
IF Staining of HeLa using 86768-1-RR
Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H4 (Lys91) antibody (86768-1-RR, Clone: 251157D9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of NIH/3T3 using 86768-1-RR
1x10^6 Trichostatin A treated NIH/3T3 cells were intracellularly stained with 0.13 ug Acetyl-Histone H4 (Lys91) Recombinant monoclonal antibody (86768-1-RR, Clone:251157D9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).
DOT BLOT Figures
Dot Blot experiment of / using 86768-1-RR
Dot blot analysis was used to confirm the specificity of 86768-1-RR Acetyl-Histone H4 (Lys91) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.
CHIP-QPCR Figures
ChIP experiment of HeLa using 86768-1-RR
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H4 (Lys91) (86768-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
Acetyl-Histone H4 (Lys91) Recombinant antibody
Catalog Number
86768-1-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 IF/ICC : 1:1000-1:4000 FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension DOT BLOT : 1:10-1:100 CHIP-QPCR : 1:10-1:100
Applications
WB, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
