CoraLite®488-conjugated Adiponectin Monoclonal antibody

Adiponectin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

5D8A7

Cat no : CL488-66239

Synonyms

ACDC, ACRP30, adiponectin, ADIPOQ, ADIPQTL1, ADPN, APM 1, APM1, GBP28, Gelatin binding protein

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Validation Data Gallery

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  IF Staining of 3T3-L1 using CL488-66239

  Immunofluorescent analysis of (-20°C Methanol) fixed 3T3-L1 cells using CoraLite®488 Adiponectin antibody (CL488-66239, Clone: 5D8A7 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: 3T3-L1 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66239 targets Adiponectin in IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Adiponectin fusion protein Ag17383
• Full Name: adiponectin, C1Q and collagen domain containing
• Calculated Molecular Weight: 244 aa, 26 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC096308
• Gene Symbol: ADIPOQ
• Gene ID (NCBI): 9370
• Conjugate: CoraLite®488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 491 nm / 516 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving insulin sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.