WB analysis using 80876-2-RR (same clone as 80876-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 80876-2-RR (Ag11509 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 80876-2-RR (Ag11509 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 80876-2-RR (same clone as 80876-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80876-2-RR (YTHDF1 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80876-2-RR (YTHDF1 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 80876-2-RR (same clone as 80876-2-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using YTHDF1 antibody (80876-2-RR, Clone: 241445B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using YTHDF1 antibody (80876-2-RR, Clone: 241445B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.
FC experiment of HEK-293 using 80876-2-RR (same clone as 80876-2-PBS)
1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug YTHDF1 Recombinant antibody (80876-2-RR, Clone:241445B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.
1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug YTHDF1 Recombinant antibody (80876-2-RR, Clone:241445B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 80876-2-RR
Biolayer interferometry (BLl) kinetic assays of 80876-2-RR against Human YTHDF1 were performed. The affinity constant is 7.23 nM.
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WB analysis using 80876-2-RR (same clone as 80876-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 80876-2-RR (Ag11509 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.
IHC Figures
IHC staining of mouse brain using 80876-2-RR (same clone as 80876-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80876-2-RR (YTHDF1 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.
IF-P Figures
IF Staining of mouse brain using 80876-2-RR (same clone as 80876-2-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using YTHDF1 antibody (80876-2-RR, Clone: 241445B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.
FC (INTRA) Figures
FC experiment of HEK-293 using 80876-2-RR (same clone as 80876-2-PBS)
1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug YTHDF1 Recombinant antibody (80876-2-RR, Clone:241445B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.
AFFINITY Figures
Affinity and Kinetic Characterization of 80876-2-RR
Biolayer interferometry (BLl) kinetic assays of 80876-2-RR against Human YTHDF1 were performed. The affinity constant is 7.23 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
YTHDF1 Recombinant antibody
Catalog Number
80876-2-PBS
Citations
-
Dilutions
Applications
WB, IHC, IF-P, FC (Intra), Indirect ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
