Alix Recombinant monoclonal antibody

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  WB analysis using 86644-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86644-1-RR (ALIX; AIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86644-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86644-1-RR against Human Alix were performed. The affinity constant is 6.12 nM.

Alix Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86644-1-RR

Clone No.251574C12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PDCD6IP, AIP1, ALG-2-interacting protein 1, ALG-2-interacting protein X, DRIP4

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, PANC-1 cells, Jurkat cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86644-1-RR targets Alix in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3074

  Product name: Recombinant human ALIX; AIP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 515-868 aa of BC020066

  Sequence: SHRDTIVLLCKPEPELNAAIPSANPAKTMQGSEVVNVLKSLLSNLDEVKKEREGLENDLKSVNFDMTSKFLTALAQDGVINEEALSVTELDRVYGGLTTKVQESLKKQEGLLKNIQVSHQEFSKMKQSNNEANLREEVLKNLATAYDNFVELVANLKEGTKFYNELTEILVRFQNKCSDIVFARKTERDELLKDLQQSIAREPSAPSIPTPAYQSSPAGGHAPTPPTPAPRTMPPTKPQPPARPPPPVLPANRAPSATAPSPVGAGTAAPAPSQTPGSAPPPQAQGPPYPTYPGYPGYCQMPMPMGYNPYAYGQYNMPYPPVYHQSPGQAPYPGPQQPSYPFPQPPQQSYYPQQ

• Full Name: programmed cell death 6 interacting protein
• Calculated Molecular Weight: 868 aa, 96 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC020066
• Gene Symbol: Alix
• Gene ID (NCBI): 10015
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8WUM4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium. The alix antibody recognizes an additional band of 75-80 kDa which has also been observed in cells and exosomes.