Angiogenin Polyclonal antibody, PBS Only

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• Angiogenin Polyclonal antibody, PBS Only (18302-1-PBS)

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  WB analysis of mouse liver using 18302-1-AP (same clone as 18302-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18302-1-AP (Angiogenin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18302-1-PBS in a different storage buffer formulation.

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  WB analysis of human milk using 18302-1-AP (same clone as 18302-1-PBS)

  human milk were subjected to SDS PAGE followed by western blot with 18302-1-AP (Angiogenin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18302-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 18302-1-AP (same clone as 18302-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18302-1-AP (Angiogenin antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18302-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 18302-1-AP (same clone as 18302-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18302-1-AP (Angiogenin antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18302-1-PBS in a different storage buffer formulation.

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  IHC staining of human Hepatocellular carcinoma using 18302-1-AP (same clone as 18302-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human Hepatocellular carcinoma slide using 18302-1-AP (Angiogenin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18302-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human Hepatocellular carcinoma using 18302-1-AP (same clone as 18302-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human Hepatocellular carcinoma slide using 18302-1-AP (Angiogenin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18302-1-PBS in a different storage buffer formulation.

Angiogenin Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 18302-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

ANG, ANG 1, ANG I, ANG1, ANGI

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Product Information

18302-1-PBS targets Angiogenin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: angiogenin, ribonuclease, RNase A family, 5
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC054880
• Gene Symbol: Angiogenin
• Gene ID (NCBI): 283
• RRID: AB_2274028
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P03950
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Angiogenin (ANG), an angiogenic ribonuclease, is a member of the vertebrate-specific, secreted RNASE superfamily. Angiogenin, originally identified as a tumor angiogenic factor, was related with the growth and metastasis of numerous tumors. Angiogenin has been proposed as a permissive factor for angiogenesis induced by other angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor, acidic fibroblast growth factor, and epidermal growth factor. Angiogenin production and secretion may be stimulated by hypoxia. Increased angiogenin serum levels have been associated with the incidence and severity of several human tumors, including HCC. It is a 17 kDa precursor which is cleaved to generate the 14 kDa mature protein.