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Angiopoietin-2 Polyclonal antibody (31499-1-AP)

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WB analysis using 31499-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 31499-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of rat lung using 31499-1-AP
IP result of anti-Angiopoietin-2 (IP:31499-1-AP, 4ug; Detection:31499-1-AP 1:3000) with rat lung tissue lysate 1200 ug.
IHC staining of mouse brain using 31499-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 31499-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 31499-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 31499-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Angiopoietin-2 Polyclonal antibody

Name: Angiopoietin-2 Polyclonal antibody
Brand: Proteintech
SKU: 31499-1-AP

Angiopoietin-2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 31499-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

Angpt2, Agpt2, Ang 2, Ang2, angiopoietin 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse lung tissue, mouse kidney tissue, rat lung tissue
Positive IP detected in	rat lung tissue
Positive IHC detected in	mouse brain tissue, mouse colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

31499-1-AP targets Angiopoietin-2 in WB, IHC, IP, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity	mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Eg0666 Product name: recombinant mouse Angiopoietin-2 protein Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 68-496 aa of NM_007426.4 Sequence: DAPLDYDDSVQRLQVLENILENNTQWLMKLENYIQDNMKKEMVEIQQNVVQNQTAVMIEIGTSLLNQTAAQTRKLTDVEAQVLNQTTRLELQLLQHSISTNKLEKQILDQTSEINKLQNKNSFLEQKVLDMEGKHSEQLQSMKEQKDELQVLVSKQSSVIDELEKKLVTATVNNSLLQKQQHDLMETVNSLLTMMSSPNSKSSVAIRKEEQTTFRDCAEIFKSGLTTSGIYTLTFPNSTEEIKAYCDMDVGGGGWTVIQHREDGSVDFQRTWKEYKEGFGSPLGEYWLGNEFVSQLTGQHRYVLKIQLKDWEGNEAHSLYDHFYLAGEESNYRIHLTGLTGTAGKISSISQPGSDFSTKDSDNDKCICKCSQMLSGGWWFDACGPSNLNGQYYPQKQNTNKFNGIKWYYWKGSGYSLKATTMMIRPADF Predict reactive species
Full Name	angiopoietin 2
Calculated Molecular Weight	56KD
Observed Molecular Weight	68 kDa
GenBank Accession Number	NM_007426.4
Gene Symbol	Angpt2
Gene ID (NCBI)	11601
RRID	AB_3742440
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity Purification
UNIPROT ID	O35608
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiopoietin-2 (ANGPT2, ANG-2) is as a regulator of vessel enlargement, and ANGPT2 inhibition prevented pathological vessel enlargement (PMID: 34758631). ANGPT2 destabilizes vessels and promotes angiogenesis through antagonism of ANGPT1 (PMID: 34758631). Ang2 was initially identified by homology with Ang1 and is expressed predominantly by endothelial cells, where it is stored in intracellular secretory granules called Weibel-Palade bodies (WPB) and promptly released after endothelium activation. Like Ang1, Ang2 binds to Tie2 receptor, but with different pathophysiological effects. While Ang1 fosters endothelial stabilization, Ang2 can antagonize Ang1, blocking Tie2 activation and leading to vessel destabilization (PMID: 31756341).

Protocols

Product Specific Protocols
IHC protocol for Angiopoietin-2 antibody 31499-1-AP	Download protocol
IP protocol for Angiopoietin-2 antibody 31499-1-AP	Download protocol
WB protocol for Angiopoietin-2 antibody 31499-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Drug Des Devel Ther Enhancing Quercetin's Potential: A Nanoliposome Delivery System for High Altitude Pulmonary Edema. Authors - Lin Lin View Article
human,mouse,rat	WB	CNS Neurosci Ther ANGPT2/Tie2 Enhances H3K18la-Mediated Macrophage M2 Polarization to Promote Endothelial Cell Proliferation in the Chronically Ischaemic Brain. Authors - Chuyang Tai View Article