Mouse Angiopoietin-2 Recombinant antibody, PBS Only

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  WB analysis using 83816-1-RR (same clone as 83816-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse lung using 83816-1-RR (same clone as 83816-1-PBS)

  IP result of anti-Angiopoietin-2 (IP:83816-1-RR, 4ug; Detection:83816-1-RR 1:1500) with mouse lung tissue lysate 1200 ug. This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 83816-1-RR (same clone as 83816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 83816-1-RR (same clone as 83816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83816-1-RR (same clone as 83816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 83816-1-RR (same clone as 83816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83816-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83816-1-RR against Mouse Angiopoietin-2 were performed. The affinity constant is below 1 pM.

Angiopoietin-2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IP, ELISA

Cat No. 83816-1-PBS

Clone No.240760D5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IP, ELISA

Synonyms

Angpt2, angiopoietin 2, Ang2, Ang 2, Agpt2

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Product Information

83816-1-PBS targets Angiopoietin-2 in WB, IHC, IP, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: angiopoietin 2
• Calculated Molecular Weight: 56KD
• Observed Molecular Weight: 68kDa
• GenBank Accession Number: NM_007426.4
• Gene Symbol: Angpt2
• Gene ID (NCBI): 11601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O35608
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Angiopoietin-2 (ANGPT2, ANG-2) is as a regulator of vessel enlargement, and ANGPT2 inhibition prevented pathological vessel enlargement (PMID: 34758631). ANGPT2 destabilizes vessels and promotes angiogenesis through antagonism of ANGPT1 (PMID: 34758631). Ang2 was initially identified by homology with Ang1 and is expressed predominantly by endothelial cells, where it is stored in intracellular secretory granules called Weibel-Palade bodies (WPB) and promptly released after endothelium activation. Like Ang1, Ang2 binds to Tie2 receptor, but with different pathophysiological effects. While Ang1 fosters endothelial stabilization, Ang2 can antagonize Ang1, blocking Tie2 activation and leading to vessel destabilization (PMID: 31756341).