Angiopoietin-2 Recombinant antibody

Angiopoietin-2 {ptg:Brand} Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 83816-1-RR
Clone No.240760D5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IP, ELISA

Angpt2, angiopoietin 2, Ang2, Ang 2, Agpt2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inmouse spleen tissue, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inmouse kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83816-1-RR targets Angiopoietin-2 in WB, IHC, IP, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein Predict reactive species
Full Name angiopoietin 2
Calculated Molecular Weight56KD
Observed Molecular Weight 68kDa
GenBank Accession NumberNM_007426.4
Gene Symbol Angpt2
Gene ID (NCBI) 11601
RRIDAB_3671402
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO35608
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiopoietin-2 (ANGPT2, ANG-2) is as a regulator of vessel enlargement, and ANGPT2 inhibition prevented pathological vessel enlargement (PMID: 34758631). ANGPT2 destabilizes vessels and promotes angiogenesis through antagonism of ANGPT1 (PMID: 34758631). Ang2 was initially identified by homology with Ang1 and is expressed predominantly by endothelial cells, where it is stored in intracellular secretory granules called Weibel-Palade bodies (WPB) and promptly released after endothelium activation. Like Ang1, Ang2 binds to Tie2 receptor, but with different pathophysiological effects. While Ang1 fosters endothelial stabilization, Ang2 can antagonize Ang1, blocking Tie2 activation and leading to vessel destabilization (PMID: 31756341).

Protocols

Product Specific Protocols
WB protocol for Angiopoietin-2 antibody 83816-1-RRDownload protocol
IHC protocol for Angiopoietin-2 antibody 83816-1-RRDownload protocol
IP protocol for Angiopoietin-2 antibody 83816-1-RRDownload protocol
Standard Protocols
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