Multi-rAb® Angiotensinogen/AGT Multi-Recombinant antibody

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  WB analysis using RMX00082

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00082 (Angiotensinogen/AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using RMX00082

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using RMX00082 (Angiotensinogen/AGT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using RMX00082

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using RMX00082 (Angiotensinogen/AGT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using RMX00082

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Angiotensinogen/AGT antibody (RMX00082) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Angiotensinogen/AGT Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00082

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AGT, Angiotensinogen, Serpin A8, SERPINA8, Alpha 1 antiproteinase

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Tested Applications

• Positive WB detected in: HepG2 cells, human plasma
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00082 targets Angiotensinogen/AGT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  FusionProtein

• Full Name: angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Calculated Molecular Weight: 485 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC011519
• Gene Symbol: AGT
• Gene ID (NCBI): 183
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P01019
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene also have been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.