Annexin IV Monoclonal antibody

Annexin IV Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1A10B5

Cat no : 60288-2-Ig

Synonyms

35-beta calcimedin, Annexin A4, Annexin IV, Annexin-4, ANXA4, Chromobindin-4, Endonexin I, Lipocortin IV



Tested Applications

Positive WB detected inCOLO 320 cells, human placenta tissue, A549 cells, human heart tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60288-2-Ig targets Annexin IV in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen Annexin IV fusion protein Ag0130
Full Name annexin A4
Calculated molecular weight 36 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC000182
Gene symbol ANXA4
Gene ID (NCBI) 307
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin IV /placental anticoagulant protein II (ANX4) is a member of the annexin or lipocortin family of calcium-dependent phospholipid binding proteins, and is located at 2p13. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 shares 45 to 59% identity with other members and possibly interacts with ATP. ANX4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells.

Protocols

Product Specific Protocols
WB protocol for Annexin IV antibody 60288-2-IgDownload protocol
IHC protocol for Annexin IV antibody 60288-2-IgDownload protocol
IF protocol for Annexin IV antibody 60288-2-IgDownload protocol
Standard Protocols
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