Annexin V Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Annexin V Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E6A8

Cat no : 66245-1-PBS

Synonyms

Anchorin CII, Annexin 5, annexin A5, Annexin V, ANX5, ANXA5, Calphobindin I, CBP I, Endonexin II, ENX2, Lipocortin V, PAP I, PP4, Thromboplastin inhibitor, VAC alpha, Vascular anticoagulant alpha

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Validation Data Gallery

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  WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

  WB result of Annexin V antibody (66245-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin V transfected HeLa cells. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

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  WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66245-1-Ig (same clone as 66245-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 66245-1-Ig (same clone as 66245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 66245-1-Ig (same clone as 66245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66245-1-Ig (same clone as 66245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66245-1-Ig (same clone as 66245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin V antibody (66245-1-Ig, Clone: 1E6A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

  Immunofluorescent analysis of HeLa cells using 66245-1-Ig (Annexin V antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

  1X10^6 HeLa cells were stained with 0.2ug Annexin V antibody (66245-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66245-1-PBS targets Annexin V in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Annexin V fusion protein Ag1538
• Full Name: annexin A5
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC001429
• Gene Symbol: ANXA5
• Gene ID (NCBI): 308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.