Annexin VI Monoclonal antibody

Annexin VI Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2H7G7

Cat no : 68086-1-Ig

Synonyms

67 kDa calelectrin, Annexin 6, annexin A6, Annexin VI, ANX6, ANXA6, Calphobindin II, CBP68, Chromobindin 20, CPB II, Lipocortin VI, p68, p70, Protein III



Tested Applications

Positive WB detected inhuman placenta tissue, LNCaP cells, HeLa cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

68086-1-Ig targets Annexin VI in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Annexin VI fusion protein Ag3263
Full Name annexin A6
Calculated molecular weight 673 aa, 76 kDa
Observed molecular weight67 kDa
GenBank accession numberBC017046
Gene symbol ANXA6
Gene ID (NCBI) 309
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.

Protocols

Product Specific Protocols
WB protocol for Annexin VI antibody 68086-1-IgDownload protocol
IF protocol for Annexin VI antibody 68086-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols