Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68316-1-Ig targets Ataxin 2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17402 Product name: Recombinant human Ataxin 2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 251-600 aa of BC114546 Sequence: GSDQRVVNGGVPWPSPCPSPSSRPPSRYQSGPNSLPPRAATPTRPPSRPPSRPSRPPSHPSAHGSPAPVSTMPKRMSSEGPPRMSPKAQRHPRNHRVSAGRGSISSGLEFVSHNPPSEAATPPVARTSPSGGTWSSVVSGVPRLSPKTHRPRSPRQNSIGNTPSGPVLASPQAGIIPTEAVAMPIPAASPTPASPASNRAVTPSSEAKDSRLQDQRQNSPAGNKENIKPNETSPSFSKAENKGISPVVSEHRKQIDDLKKFKNDFRLQPSSTSESMDQLLNKNREGEKSRDLIKDKIEPSAKDSFIENSSSNCTSGSSKPNSPSISPSILSNTEHKRGPEVTSQGVQTSS Predict reactive species |
| Full Name | ataxin 2 |
| Calculated Molecular Weight | 1313 aa, 140 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | BC114546 |
| Gene Symbol | ATXN2 |
| Gene ID (NCBI) | 6311 |
| RRID | AB_3085052 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Caprylic acid/ammonium sulfate precipitation |
| UNIPROT ID | Q99700 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATXN2 contains a repeat structure with 22 or 23 triplets coding for glutamine and the (CAG)8CAA(CAG)4CAA(CAG)8 sequence; expansion of this domain to a size ≥34 triplets with a pure CAG sequence primarily causes autosomal dominant SCA2 [PMID:18418684], while ATXN2 expansions with CAA interruptions were observed as the cause of Levo-dopa responsive Parkinson's disease [PMID:10668726]. ATXN2 expansions associated with ALS were reported to be interrupted by at least one CAA triplet [PMID:21537950]
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Ataxin 2 antibody 68316-1-Ig | Download protocol |
| IHC protocol for Ataxin 2 antibody 68316-1-Ig | Download protocol |
| WB protocol for Ataxin 2 antibody 68316-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Ataxin 2 Monoclonal antibody (1E8A2), catalog number 68316-1-Ig, has a total of 2 citations. One published citation appears in Nature Cell Biology (2026), addressing the role of stress granules in restraining ferroptosis through ferritin sequestration. The associated research fields include cell biology, cell death (ferroptosis), stress granule biology, and iron metabolism. The antibody is a mouse IgM monoclonal targeting human, mouse, and rat Ataxin 2, validated for WB, IHC, IF, and ELISA applications.
| Species | Application | Title |
|---|---|---|
Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The reviewer used it for Western Blot and noted that the antibody is sensitive enough to work with a fluorescent secondary antibody, describing the overall performance as fantastic.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Aditya (Verified Customer) (10-08-2024) | sensitive enough to use flourescent secondary. fantastic
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