Ataxin 2 Monoclonal antibody

Ataxin 2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E8A2

Cat no : 68316-1-Ig

Synonyms

ataxin 2, ATX2, ATXN2, FLJ46772, SCA2, TNRC13

---

Validation Data Gallery

• 

  WB analysis using 68316-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68316-1-Ig (Ataxin 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 68316-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68316-1-Ig (Ataxin 2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 68316-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68316-1-Ig (Ataxin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 68316-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Ataxin 2 antibody (68316-1-Ig, Clone: 1E8A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of rat brain using 68316-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Ataxin 2 antibody (68316-1-Ig, Clone: 1E8A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68316-1-Ig targets Ataxin 2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: Ataxin 2 fusion protein Ag17402
• Full Name: ataxin 2
• Calculated Molecular Weight: 1313 aa, 140 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC114546
• Gene Symbol: ATXN2
• Gene ID (NCBI): 6311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATXN2 contains a repeat structure with 22 or 23 triplets coding for glutamine and the (CAG)8CAA(CAG)4CAA(CAG)8 sequence; expansion of this domain to a size ≥34 triplets with a pure CAG sequence primarily causes autosomal dominant SCA2 [PMID:18418684], while ATXN2 expansions with CAA interruptions were observed as the cause of Levo-dopa responsive Parkinson's disease [PMID:10668726]. ATXN2 expansions associated with ALS were reported to be interrupted by at least one CAA triplet [PMID:21537950]