Ataxin-3/ATXN3 Recombinant monoclonal antibody, PBS Only

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• Ataxin-3/ATXN3 Recombinant monoclonal antibody, PBS Only (87817-1-PBS)

Ataxin-3/ATXN3 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 87817-1-PBS

Clone No.253467D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ATX3,ATXN3, ATXN3, AT3, ataxin 3, Ataxin-3

• 

  WB analysis using 87817-1-RR (same clone as 87817-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87817-1-RR (Ataxin-3/ATXN3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87817-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 87817-1-RR (same clone as 87817-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87817-1-RR (MU154079-4(ATXN3) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87817-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 87817-1-RR (same clone as 87817-1-PBS)

  WB result of Ataxin-3/ATXN3 antibody (87817-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ataxin-3/ATXN3 transfected MCF-7 cells. This data was developed using the same antibody clone with 87817-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human bowen disease using 87817-1-RR (same clone as 87817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease tissue slide using 87817-1-RR (Ataxin-3/ATXN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87817-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human bowen disease using 87817-1-RR (same clone as 87817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease tissue slide using 87817-1-RR (Ataxin-3/ATXN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87817-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 87817-1-RR

  Biolayer interferometry (BLI) kinetic assays of 87817-1-RR against Human Ataxin-3/ATXN3 were performed. The affinity constant is below 1 pM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

87817-1-PBS targets Ataxin-3/ATXN3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag41832

  Product name: Recombinant human MU154079-4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-361 aa of

  Sequence: MSKIKMESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDLPDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGSGMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMTQTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQGDLSGQSSHPCERPATSSGALGSDLGDAMSEEDMLQAAVTMSLETVRNDLKTEGKKHHHHHHHH

• Full Name: ataxin 3
• Observed Molecular Weight: 35-42 kDa
• Gene Symbol: ATXN3
• Gene ID (NCBI): 4287
• RRID: AB_3745767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P54252
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.