Ataxin-3/ATXN3 Recombinant monoclonal antibody

Ataxin-3/ATXN3 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 87817-1-RR

Clone No.253467D4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ATX3,ATXN3, ATXN3, AT3, ataxin 3, Ataxin-3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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-/ -

Freight/Packing: -

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Filter: AllWBIHCAffinity

WB analysis using 87817-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87817-1-RR (Ataxin-3/ATXN3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 87817-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87817-1-RR (MU154079-4(ATXN3) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 87817-1-RR
WB result of Ataxin-3/ATXN3 antibody (87817-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ataxin-3/ATXN3 transfected MCF-7 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human bowen disease using 87817-1-RR
Immunohistochemical analysis of paraffin-embedded human bowen disease tissue slide using 87817-1-RR (Ataxin-3/ATXN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human bowen disease using 87817-1-RR
Immunohistochemical analysis of paraffin-embedded human bowen disease tissue slide using 87817-1-RR (Ataxin-3/ATXN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 87817-1-RR
Biolayer interferometry (BLI) kinetic assays of 87817-1-RR against Human Ataxin-3/ATXN3 were performed. The affinity constant is below 1 pM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, MCF-7 cells, Neuro-2a cells, rat brain tissue, mouse brain tissue
Positive IHC detected in	human bowen disease tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87817-1-RR targets Ataxin-3/ATXN3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag41832 Product name: Recombinant human MU154079-4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-361 aa of Sequence: MSKIKMESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDLPDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGSGMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMTQTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQGDLSGQSSHPCERPATSSGALGSDLGDAMSEEDMLQAAVTMSLETVRNDLKTEGKKHHHHHHHH Predict reactive species
Full Name	ataxin 3
Observed Molecular Weight	35-42 kDa
Gene Symbol	ATXN3
Gene ID (NCBI)	4287
RRID	AB_3745767
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P54252
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.