AuthentiKine™ Human IFN-beta ELISA Kit

Sensitivity

0.76 pg/ml

Range

7.8-500 pg/mL

Reactivity

Human

Cat no : KE00195

Synonyms

Fibroblast interferon, IFB, IFF, IFN beta, IFNB, IFNB1, Interferon beta, interferon, beta 1, fibroblast



Product Information

KE00195 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFN-beta ELISA kit is to be used to detect and quantify protein levels of endogenous IFN-beta. The assay recognizes human IFN-beta. An antibody specific for IFN-beta has been pre-coated onto the microwells. The IFN-beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IFN-beta is added to detect the captured IFN-beta protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name AuthentiKine™ Human IFN-beta ELISA Kit
Tests 1 X 96 well plate
Sample type Human Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.76 pg/ml
Range 7.8-500 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human Plasma8972-107
Cell culture supernatants 8478-96

IntraAssay

Samplenmean ( pg/ml)SDCV%
120244.55.22.1
22061.61.72.8
32014.91.38.8

InterAssay

Samplenmean ( pg/ml)SDCV%
124244.76.32.6
22464.52.03.1
32416.51.69.6

Background Information

Interferon beta (IFN-beta) is a cytokine that is naturally produced by the immune system in response to biological and chemical stimuli. It signals by binding to the heterodimeric type I IFN receptor composed of the IFNAR1 and IFNAR2 chains, and regulates the expression of a plethora of genes by means of the classical JAK/STAT and other pathways. Interferon beta (IFN-beta) has been shown in several clinical trials to have efficacy in multiple sclerosis. Interferon beta gene therapy is expected to become widely available for clinical use in cancer patients, and this new strategy might be extended to molecular targeting therapy, or used in combination with cell therapy or other therapies.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms Fibroblast interferon, IFB, IFF, IFN beta, IFNB, IFNB1, Interferon beta, interferon, beta 1, fibroblast