AuthentiKine™ Human IL-23 ELISA Kit
Sensitivity
0.15 pg/mL
Range
15.6-1000 pg/mL
Reacted Species
Human
Cat no : KE00192
Synonyms
IL 23, IL 23 A, IL 23 subunit alpha, IL 23A, IL 23p19, IL23A, IL23P19, Interleukin 23 subunit alpha, Interleukin 23 subunit p19, P19, SGRF
Validation Data Gallery
Product Information
KE00192 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-23 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-23. The assay recognizes human IL-23. An antibody specific for IL-23 has been pre-coated onto the microwells. The IL-23 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL-23 is added to detect the captured IL-23 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human IL-23 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.15 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reacted Species | Human |
| Tested applications | Sandwich ELISA |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| serum | 97 | 74-122 |
| cell culture supernatants | 103 | 88-124 |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 283.2 | 19.6 | 6.9 |
| 2 | 20 | 603.3 | 53.3 | 8.8 |
| 3 | 20 | 1065.1 | 39.4 | 3.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 287.7 | 26.9 | 9.3 |
| 2 | 24 | 580.1 | 48.6 | 8.4 |
| 3 | 24 | 1059.3 | 76.0 | 7.2 |
Background Information
Interleukin 23 (IL23) is a member of the IL12 cytokine family and composed of two subunits, IL12p40 and IL23p19. It is produced by antigen presenting cells and has been shown to promote the production and survival of a distinct lineage of T-cells called TH17 cells. A functional receptor for IL23 (the IL23 receptor) has been identified and is composed of IL12Rβ1 and IL23R. IL23 is expressed chiefly by the macrophages and DCs. The IL-23R is found on memory T cells, NKT cells, macrophages, DCs, and naive T cells upon activation by TGF-βand IL6. The main biological effects of IL23 identified initially consist of stimulation of antigen presentation by DCs, T cell differentiation to Th17 cells, and production of interferon-γ (IFN-γ). IL23 acts also as an end-stage effector cytokine through direct action on macrophages.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms | IL 23, IL 23 A, IL 23 subunit alpha, IL 23A, IL 23p19, IL23A, IL23P19, Interleukin 23 subunit alpha, Interleukin 23 subunit p19, P19, SGRF |