AuthentiKine™ Human IL-27 ELISA Kit

Sensitivity

0.1 pg/mL

Range

78.1-5000 pg/mL

Reacted Species

Human

Cat no : KE00182

Synonyms

IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha, p28



Product Information

KE00182 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL27 ELISA kit is to be used to detect and quantify protein levels of endogenous IL27. The assay recognizes human IL27. An antibody specific for IL27 has been pre-coated onto the microwells. The IL27 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL27 is added to detect the captured IL27 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm .

Product name AuthentiKine™ Human IL-27 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma
Assay type Sandwich
Sensitivity 0.1 pg/mL
Range 78.1-5000 pg/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plasma99%80%-114%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1202555.961.32.4
220383.89.62.5
32072.76.08.2

InterAssay

Samplenmean ( pg/mL)SDCV%
1242667.379.73.0
224411.710.22.5
32494.97.57.9

Background Information

IL27, a member of IL12/IL23 heterodimeric family of cytokines, has pleiotropic properties that can enhance or limit immune responses. IL27 is a heterodimeric cytokine composed of two subunits: IL27a and IL27b. IL27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL10 producing type 1 regulatory T cells. IL27 is a potentially promising cytokine for therapeutic approaches on various human diseases.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha, p28