AuthentiKine™ Human M-CSF ELISA Kit

Sensitivity

1.1 pg/mL

Range

15.6-1000 pg/mL

Reacted Species

Human

Cat no : KE00184

Synonyms

CSF 1, CSF1, Lanimostim, MCSF, M-CSF



Product Information

KE00184 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The M-CSF ELISA kit is to be used to detect and quantify protein levels of endogenous M-CSF. The assay recognizes human M-CSF. An antibody specific for M-CSF has been pre-coated onto the microwells. The M-CSF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for M-CSF is added to detect the captured M-CSF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name AuthentiKine™ Human M-CSF ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Urine, Saliva
Assay type Sandwich
Sensitivity 1.1 pg/mL
Range 15.6-1000 pg/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plasma8271-88
Cell culture supernatants8580-94
Urine9589-101
Saliva9284-107

IntraAssay

Samplenmean ( pg/mL)SDCV%
120207.3 5.52.7
220433.8 8.82.0
320797.3 51.76.5

InterAssay

Samplenmean ( pg/mL)SDCV%
124214.6 6.93.2
224475.3 22.14.7
324880.948.0 5.0

Background Information

M-CSF, also called colony-stimulating factor-1 or CSF-1), is a haematopoietic growth factor, which controls survival, proliferation, differentiation and function of mononuclear phagocytes. It also plays a role in bone metabolism, fertility, pregnancy and inflammatory processes. M-CSF may be expressed on the surface of the cell as a membrane-spanning glycoprotein or secreted into circumstance. M-CSF was identified as an 84-kDa homodimeric glycoprotein found in healthy human urine, and is present in the peripheral blood and urine. M-CSF serum levels were elevated in pre-transplant haemodialysis patients, so it might be a specific marker of Kidney Allograft Rejection.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms CSF 1, CSF1, Lanimostim, MCSF, M-CSF