AuthentiKine™ Human FLT3 Ligand ELISA Kit

Sensitivity

0.15 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Human

Cat no : KE00197

Synonyms

FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine



Product Information

KE00197 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FLT3 Ligand ELISA kit is to be used to detect and quantify protein levels of endogenous FLT3 Ligand. The assay recognizes human FLT3 Ligand. An antibody specific for FLT3 Ligand has been pre-coated onto the microwells. TheFLT3 Ligand protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for FLT3 Ligand is added to detect the captured FLT3 Ligand protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name AuthentiKine™ Human FLT3 Ligand ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.15 pg/mL
Range 15.6-1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plasma8981-105
Cell culture supernatants 8976-101

IntraAssay

Samplenmean ( pg/mL)SDCV%
120511.813.42.6
220124.72.92.3
32028.21.24.3

InterAssay

Samplenmean ( pg/mL)SDCV%
124517.117.33.3
224123.44.63.7
32428.51.55.1

Background Information

FLT3 Ligand is a member of a small family of growth factors that stimulate the proliferation of hematopoietic cell. FLT3 ligand is known to induce profound expansion of dendritic cells (DCs) in the peripheral lymphoid organs, and FLT3 ligand-induced DCs have an immature phenotype. Its receptor, FLT3, is expressed on myeloid, lymphoid and dendritic cell progenitors and is considered an important growth and differentiation factor for several hematopoietic lineages. Activating mutations of FLT3 are frequently found in acute myeloid leukemia (AML) patients and associated with a poor clinical prognosis. Serum FLT3 Ligand is a biomarker of progenitor cell mass and prognosis in acute myeloid leukemia.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine