AuthentiKine™ Human FLT3 Ligand ELISA Kit
Cat no : KE00197
Synonyms
FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine
Validation Data Gallery
Product Information
KE00197 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FLT3 Ligand ELISA kit is to be used to detect and quantify protein levels of endogenous FLT3 Ligand. The assay recognizes human FLT3 Ligand. An antibody specific for FLT3 Ligand has been pre-coated onto the microwells. The FLT3 Ligand protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for FLT3 Ligand is added to detect the captured FLT3 Ligand protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human FLT3 Ligand ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.15 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2323 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 89 | 81-105 |
| Cell culture supernatants | 89 | 76-101 |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 511.8 | 13.4 | 2.6 |
| 2 | 20 | 124.7 | 2.9 | 2.3 |
| 3 | 20 | 28.2 | 1.2 | 4.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 517.1 | 17.3 | 3.3 |
| 2 | 24 | 123.4 | 4.6 | 3.7 |
| 3 | 24 | 28.5 | 1.5 | 5.1 |
Background Information
FLT3 Ligand is a member of a small family of growth factors that stimulate the proliferation of hematopoietic cell. FLT3 ligand is known to induce profound expansion of dendritic cells (DCs) in the peripheral lymphoid organs, and FLT3 ligand-induced DCs have an immature phenotype. Its receptor, FLT3, is expressed on myeloid, lymphoid and dendritic cell progenitors and is considered an important growth and differentiation factor for several hematopoietic lineages. Activating mutations of FLT3 are frequently found in acute myeloid leukemia (AML) patients and associated with a poor clinical prognosis. Serum FLT3 Ligand is a biomarker of progenitor cell mass and prognosis in acute myeloid leukemia.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.