AuthentiKine™ Human IL-1 alpha ELISA Kit
Cat no : KE00268
Synonyms
interleukin 1, alpha, IL1 ALPHA, IL 1A, IL 1 alpha, Interleukin 1 alpha, IL1, Hematopoietin 1, IL1A, IL1F1
Validation Data Gallery
Product Information
KE00268 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-1 alpha ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1 alpha. The assay recognizes human IL-1 alpha. An antibody specific for IL-1 alpha has been pre-coated onto the microwells. The IL-1 alpha protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL-1 alpha is added to detect the captured IL-1 alpha protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human IL-1 alpha ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, plasma, cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.2 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3552 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 87% | 80%-97% |
| Cell culture supernatants | 92% | 81%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 232.2 | 11.6 | 5.0 |
| 2 | 20 | 60.5 | 3.7 | 6.1 |
| 3 | 20 | 15.3 | 0.8 | 5.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 233.1 | 8.6 | 3.7 |
| 2 | 24 | 57.9 | 2.6 | 4.5 |
| 3 | 24 | 14.3 | 0.8 | 5.4 |
Background Information
Interleukin-1α (IL-1 alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | interleukin 1, alpha, IL1 ALPHA, IL 1A, IL 1 alpha, Interleukin 1 alpha, IL1, Hematopoietin 1, IL1A, IL1F1 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Photochem Photobiol Sci Protective effect and mechanism research of Phyllanthus emblica Linn. fruit extract on UV-induced photodamage in keratinocytes |