AuthentiKine™ Human IL-28B ELISA Kit
Cat no : KE00235
Synonyms
IL28B, IL-28B/ IFNL3, Cytokine Zcyto22, IFN lambda 3, IFN lambda 4
Validation Data Gallery
Product Information
KE00235 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-28B ELISA kit is to be used to detect and quantify protein levels of endogenous IL-28B. The assay recognizes human IL-28B. An antibody specific for IL-28B has been pre-coated onto the microwells. The IL-28B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL-28B is added to detect the captured IL-28B protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human IL-28B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants , Urine |
| Assay type | Sandwich |
| Sensitivity | 2.1 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 282617 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Plasma | 101% | 95%-112% |
| Cell culture supernatants | 111% | 98%-123% |
| Urine | 96% | 90%-102% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 423.9 | 7.1 | 1.7 |
| 2 | 20 | 103.5 | 2.6 | 2.6 |
| 3 | 20 | 22.3 | 0.4 | 1.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 427.6 | 9.0 | 2.1 |
| 2 | 24 | 101.5 | 1.1 | 1.1 |
| 3 | 24 | 22.8 | 0.5 | 2.2 |
Background Information
IL-28B, also named as IFNL3, is a cytokine distantly related to type I interferons and the IL-10 family. IL-28B, interleukin 28A (IL-28A), and interleukin 29 (IL-29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL-10RB) and interleukin 28 receptor, alpha (IL-28RA). IL-28B was identified as a key regulator of B- and T-cell vaccine responses against influenza.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL28B, IL-28B/ IFNL3, Cytokine Zcyto22, IFN lambda 3, IFN lambda 4 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
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