AuthentiKine™ Human Thrombopoietin ELISA Kit
Cat no : KE00267
Synonyms
THPO,thrombopoietin,TPO
Validation Data Gallery
Product Information
KE00267 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Thrombopoietin(TPO) ELISA kit is to be used to detect and quantify protein levels of endogenous TPO. The assay recognizes human TPO. An antibody specific for human TPO has been pre-coated onto the microwells. The human TPO protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TPO is added to detect the captured human TPO protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human Thrombopoietin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 2.6 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7066 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 90% | 75%-124% |
| Cell culture supernatants | 93% | 86%-104% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 543.1 | 29.2 | 5.4 |
| 2 | 20 | 126.5 | 4.5 | 3.6 |
| 3 | 20 | 32.4 | 2.0 | 6.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 458.0 | 32.7 | 7.2 |
| 2 | 24 | 120.2 | 5.3 | 4.4 |
| 3 | 24 | 32.6 | 1.7 | 5.1 |
Background Information
Thrombopoietin (TPO) also known as megakaryocyte growth and development factor (MGDF), is a protein in human. The protein is a glycoprotein hormone produced by the liver and kidney, regulating the differentiation of megakaryocytes and platelets. While platelet and megakaryocyte concentrations have a negative feedback on TPO levels. It is also a humoral growth factor necessary for megakaryocyte proliferation and maturation, thrombopoiesis. TPO is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene and one of the Class I hematopoietic cytokines. TPO promotes apoptosis of newly generated neurons in brain, an effect counteracted by Erythropoietin and neurotrophins.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | THPO,thrombopoietin,TPO |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.