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CoraLite®568-conjugated B23/NPM1 Monoclonal antibody

B23/NPM1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®568 Fluorescent Dye

CloneNo.

4F12A3

Cat no : CL568-60096

Synonyms

B23, B23/NPM1, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin



Tested Applications

Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60096-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

CL568-60096 targets B23/NPM1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen B23/NPM1 fusion protein Ag7415
Full Name nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Calculated molecular weight 33 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC002398
Gene symbol NPM1
Gene ID (NCBI) 4869
Conjugate CoraLite®568 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

Protocols

Product Specific Protocols
IF protocol for CL568 B23/NPM1 antibody CL568-60096Download protocol
FC protocol for CL568 B23/NPM1 antibody CL568-60096Download protocol
Standard Protocols
Click here to view our Standard Protocols