B3GALT6 Polyclonal antibody

B3GALT6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 55049-1-AP

Synonyms

B3GALT6, Beta 1,3 GalTase 6, Beta3Gal T6, beta3GalT6, GAG GalTII, Galactosyltransferase II

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Validation Data Gallery

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  WB analysis of mouse brain using 55049-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55049-1-AP (B3GALT6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55049-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55049-1-AP (B3GALT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 55049-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 55049-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 55049-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 55049-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 55049-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 55049-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

55049-1-AP targets B3GALT6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 6
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: NM_080605
• Gene Symbol: B3GALT6
• Gene ID (NCBI): 126792
• RRID: AB_10888634
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B3GALT6 belongs to the glycosyltransferase 31 family. It transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. B3GALT6 has a preference for galactose-beta-1,4-xylose that is found in the linker region of glycosaminoglycans, such as heparan sulfate and chondroitin sulfate. It has no activity towards substrates with terminal glucosamine or galactosamine residues. The antibody is specific to B3GALT6.

Publications

Species	Application	Title
human	WB	Nat Microbiol; Sulfated glycosaminoglycans are host epithelial cell targets of the Candida albicans toxin candidalysin; Authors - Jianfeng Lin
human	WB	bioRxiv; A genome-scale screen identifies sulfated glycosaminoglycans as pivotal in epithelial cell damage by Candida albicans; Authors - Jianfeng Lin ; KD Validated