B3GNT6 Polyclonal antibody, PBS Only

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• B3GNT6 Polyclonal antibody, PBS Only (21291-1-PBS)

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  WB analysis using 21291-1-AP (same clone as 21291-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21291-1-AP (B3GNT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21291-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 21291-1-AP (same clone as 21291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21291-1-AP (B3GNT6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21291-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 21291-1-AP (same clone as 21291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21291-1-AP (B3GNT6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21291-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 21291-1-AP (same clone as 21291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21291-1-AP (B3GNT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21291-1-PBS in a different storage buffer formulation.

B3GNT6 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 21291-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

BGnT-6, BGnT 6, Beta-1,3-N-acetylglucosaminyltransferase 6, B3Gn-T6, B3Gn T6

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Product Information

21291-1-PBS targets B3GNT6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15825

  Product name: Recombinant human B3GNT6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 102-343 aa of BC103910

  Sequence: PLLWDAPAKCAGGRGVFLLLAVKSAPEHYERRELIRRTWGQERSYGGRPVRRLFLLGTPGPEDEARAERLAELVALEAREHGDVLQWAFADTFLNLTLKHLHLLDWLAARCPHARFLLSGDDDVFVHTANVVRFLQAQPPGRHLFSGQLMEGSVPIRDSWSKYFVPPQLFPGSAYPVYCSGGGFLLSGPTARALRAAARHTPLFPIDDAYMGMCLERAGLAPSGHEGIRPFGVQLPGAQQSS

• Full Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 (core 3 synthase)
• Calculated Molecular Weight: 384 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC103910
• Gene Symbol: B3GNT6
• Gene ID (NCBI): 192134
• RRID: AB_2878834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZMB0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The B3GNT6 protein, also known as core 3 synthase, is a member of the O-GlcNAc transferase (OGT) family and adds an N-acetylglucosamine to N-acetylgalactosamine-modifed serine or threonine. The B3GNT6 protein is responsible for the formation of the core 3 structure of O-glycans, which are important components of mucin-type glycoproteins. This process is known as the protein O-GlcNAcylation modifcation. B3GNT6 expression has been reported to be largely downregulated in gastric and colorectal cancers. B3GNT6 has 3 isoforms with the molecular mass of 29 and 43 kDa. (PMID: 35387659)