B4GALT3 Polyclonal antibody, PBS Only

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• B4GALT3 Polyclonal antibody, PBS Only (11041-1-PBS)

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  WB analysis using 11041-1-AP (same clone as 11041-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11041-1-AP (B4GALT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 11041-1-AP (same clone as 11041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 11041-1-AP (same clone as 11041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11041-1-AP (same clone as 11041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11041-1-AP (same clone as 11041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 11041-1-AP (same clone as 11041-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 11041-1-AP (B4GALT3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 11041-1-AP (same clone as 11041-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using B4GALT3 antibody (11041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

B4GALT3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 11041-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

b4Gal T3, b4Gal-T3, Beta 1,4 GalTase 3, Beta-1,4-galactosyltransferase 3, Beta-1,4-GalTase 3

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Product Information

11041-1-PBS targets B4GALT3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1487

  Product name: Recombinant human B4GALT3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-321 aa of BC009985

  Sequence: MLRRLLERPCTLALLVGSQLAVMMYLSLGGFRSLSALFGRDQGPTFDYSHPRDVYSNLSHLPGAPGGPPAPQGLPYCPERSPLLVGPVSVSFSPVPSLAEIVERNPRVEPGGRYRPAGCEPRSRTAIIVPHRAREHHLRLLLYHLHPFLQRQQLAYGIYVIHQAGNGTFNRAKLLNVGVREALRDEEWDCLFLHDVDLLPENDHNLYVCDPRGPRHVAVAMNKFGYSLPYPQYFGGVSALTPDQYLKMNGFPNEYWGWGGEDDDIATRVRLAGMKISRPPTSVGHYKMVKHRGDKGNEENPHRFDLLVRTQNSWTQDGMNS

• Full Name: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC009985
• Gene Symbol: B4GALT3
• Gene ID (NCBI): 8703
• RRID: AB_2290072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60512
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

B4GALT3 is an enzyme responsible for the generation of poly-N-acetyllactosamine. The B4GALTs transfer galactose from UDP-Gal to N-acetylglucosamine (GlcNAc)-terminated oligosaccharides on N-glycan, O-glycan, or glycolipid to form N-acetyllactosamin (PMID: 23444218). B4GALT3 has been found to regulate cancer cell invasion (PMID: 25659296). B4GALT3 knockdown increased cell migration, invasion and the activation of β1 integrin and its downstream signaling (PMID: 24403309).