Tested Applications
| Positive WB detected in | mouse heart tissue, mouse kidney tissue, HEK-293 cells, mouse brain tissue |
| Positive IHC detected in | human colon tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20148-1-AP targets B4GALT6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13686 Product name: Recombinant human B4GALT6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-382 aa of BC074884 Sequence: MSVLRRMMRVSNRSLLAFIFFFSLSSSCLYFIYVAPGIANTYLFMVQARGIMLRENVKTIGHMIRLYTNKNSTLNGTDYPEGNNSSDYLVQTTTYLPENFTYSPYLPCPEKLPYMRGFLNVNVSEVSFDEIHQLFSKDLDIEPGGHWRPKDCKPRWKVAVLIPFRNRHEHLPIFFLHLIPMLQKQRLEFAFYVIEQTGTQPFNRAMLFNVGFKEAMKDSVWDCVIFHDVDHLPENDRNYYGCGEMPRHFAAKLDKYMYILPYKEFFGGVSGLTVEQFRKINGFPNAFWGWGGEDDDLWNRVHYAGYNVTRPEGDLGKYKSIPHHHRGEVQFLGRYKLLRYSKERQYIDGLNNLIYRPKILVDRLYTNISVNLMPELAPIEDY Predict reactive species |
| Full Name | UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6 |
| Calculated Molecular Weight | 382 aa, 45 kDa |
| Observed Molecular Weight | 45-60 kDa |
| GenBank Accession Number | BC074884 |
| Gene Symbol | B4GALT6 |
| Gene ID (NCBI) | 9331 |
| RRID | AB_10665357 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBX8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Beta-1,4-galactosyltransferase 6 (B4GALT6) is a member of the beta 4-galactosyltransferase family that catalyzes the biosynthesis of glycosphingolipids in UDP-dependent manner (PMID: 23744354). B4GALT6 in astrocytes boosts CNS inflammation and promote neurodegeneration during chronic CNS inflammation (PMID: 25216636).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for B4GALT6 antibody 20148-1-AP | Download protocol |
| IHC protocol for B4GALT6 antibody 20148-1-AP | Download protocol |
| WB protocol for B4GALT6 antibody 20148-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The B4GALT6 Polyclonal antibody (catalog no. 20148-1-AP) has 6 total citations. These appear in Nature Medicine, Cell Metabolism, Nature Communications, Biochimica et Biophysica Acta – Molecular and Cell Biology of Lipids, World Journal of Psychiatry, and Research Square. The associated research fields span chronic CNS inflammation/astrocyte activation, obesity-related breast cancer, cholestasis and bile acid metabolism, sphingolipid metabolism in colon tumors, B4GALT6 brain region/cell specificity in depression, and DNA methylation in frontal cortex. Applications include IF, IHC, and WB.
| Species | Application | Title |
|---|---|---|
Nat Med Regulation of astrocyte activation by glycolipids drives chronic CNS inflammation. | ||
Cell Metab Adipocyte-derived glutathione promotes obesity-related breast cancer by regulating the SCARB2-ARF1-mTORC1 complex | ||
Nat Commun Targeting the ceramidase ACER3 attenuates cholestasis in mice by mitigating bile acid overload via unsaturated ceramide-mediated LXRβ signaling transduction | ||
Biochim Biophys Acta Mol Cell Biol Lipids Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors. | ||
World J Psychiatry Brain region and cell specificity of B4GALT6 in mice with depressive-like behavior: A pilot study. | ||
Res Sq Duration of contact sports play associated with aberrant DNA methylation in human frontal cortex. |
