B4GALT6 Recombinant monoclonal antibody

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• B4GALT6 Recombinant monoclonal antibody (87222-2-RR)

B4GALT6 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87222-2-RR

Clone No.251395A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

b4Gal-T6, Beta-1,4-galactosyltransferase 6, Beta-1,4-GalTase 6, Beta4Gal-T6, EC:2.4.1.-

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  WB analysis using 87222-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87222-2-RR (B4GALT6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87222-2-RR targets B4GALT6 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13686

  Product name: Recombinant human B4GALT6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-382 aa of BC074884

  Sequence: MSVLRRMMRVSNRSLLAFIFFFSLSSSCLYFIYVAPGIANTYLFMVQARGIMLRENVKTIGHMIRLYTNKNSTLNGTDYPEGNNSSDYLVQTTTYLPENFTYSPYLPCPEKLPYMRGFLNVNVSEVSFDEIHQLFSKDLDIEPGGHWRPKDCKPRWKVAVLIPFRNRHEHLPIFFLHLIPMLQKQRLEFAFYVIEQTGTQPFNRAMLFNVGFKEAMKDSVWDCVIFHDVDHLPENDRNYYGCGEMPRHFAAKLDKYMYILPYKEFFGGVSGLTVEQFRKINGFPNAFWGWGGEDDDLWNRVHYAGYNVTRPEGDLGKYKSIPHHHRGEVQFLGRYKLLRYSKERQYIDGLNNLIYRPKILVDRLYTNISVNLMPELAPIEDY

• Full Name: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6
• Calculated Molecular Weight: 382 aa, 45 kDa
• Observed Molecular Weight: 45-60 kDa
• GenBank Accession Number: BC074884
• Gene Symbol: B4GALT6
• Gene ID (NCBI): 9331
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UBX8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B4GALT6 encodes for a type II membrane-bound glycoprotein, which plays a crucial role in lactosylceramide synthesis by transferring galactose from UDP-galactose to glucosylceramide. B4GALT6 in astrocytes boosts CNS inflammation and promote neurodegeneration during chronic CNS inflammation (PMID: 25216636). Abnormal interactions of glycosyltransferases/glycosidases such as B4GALT6 can cause imbalances in key glycosylation modifications, thereby driving the disease process through a triple cascade reaction (PMID: 41112602).