CD80 Polyclonal antibody, PBS Only

• 

  WB analysis using 14292-1-AP (same clone as 14292-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14292-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human peripheral blood leukocyte using 14292-1-AP (same clone as 14292-1-PBS)

  Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14292-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 14292-1-AP (same clone as 14292-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14292-1-PBS in a different storage buffer formulation.

CD80 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 14292-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Activation B7 1 antigen, Activation B7-1 antigen, B7, B7 1, B7-1

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Product Information

14292-1-PBS targets CD80 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5615

  Product name: Recombinant human B7-1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 36-228 aa of BC042665

  Sequence: IHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQT

• Full Name: CD80 molecule
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC042665
• Gene Symbol: CD80
• Gene ID (NCBI): 941
• RRID: AB_10640809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P33681
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)