B7-H4 Recombinant monoclonal antibody

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  WB analysis using 82850-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82850-1-RR (B7‑H4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 82850-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82850-1-RR (B7-H4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 82850-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82850-1-RR (B7-H4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

B7-H4 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 82850-1-RR

Clone No.4C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

VTCN1, B7‑H4, B7H4, B7h.5, B7 homolog 4

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Tested Applications

• Positive WB detected in: HeLa cells, SKOV-3 cells, BGC-823 cells, SK-BR-3 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82850-1-RR targets B7-H4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: V-set domain containing T cell activation inhibitor 1
• Calculated Molecular Weight: 282 aa, 31 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: NM_024626
• Gene Symbol: B7-H4
• Gene ID (NCBI): 79679
• RRID: AB_3670576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7Z7D3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B7-H4, also named VTCN1, B7X, or B7S1, is a single-pass type I membrane protein, which contains two immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7-H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7-H4 is over-expressed in many cancers, including breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.