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Anti-Rat B7-H4 Rabbit Recombinant Antibody, PBS Only (98552-2-PBS)

Anti-Rat B7-H4 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Rat B7-H4 Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98552-2-PBS-1MG

B7-H4 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98552-2-PBS

Clone No. 250392E12

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Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

Synonyms

B7H4, B7S1, B7x, V-set domain-containing T-cell activation inhibitor 1, Vtcn1

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

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Filter: AllFC

FC experiment of Transfected HEK-293T using 98552-2-RR (same clone as 98552-2-PBS)
1x10^6 HEK-293T cells (left) or B7-H4 transfected HEK-293T cells (right) were surface stained with 0.25 ug Anti-Rat B7-H4 Rabbit RecAb (98552-2-RR, Clone: 250392E12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98552-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Transfected HEK-293T using 98552-2-RR (same clone as 98552-2-PBS)
1x10^6 HEK-293T cells (left) or B7-H4 transfected HEK-293T cells (right) were surface stained with 0.25 ug Anti-Rat B7-H4 Rabbit RecAb (98552-2-RR, Clone:250392E12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98552-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1x10^6 HEK-293T cells (left) or B7-H4 transfected HEK-293T cells (right) were surface stained with 0.25 ug Anti-Rat B7-H4 Rabbit RecAb (98552-2-RR, Clone:250392E12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98552-2-PBS in a different storage buffer formulation.

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

98552-2-PBS targets B7-H4 in FC applications and shows reactivity with rat samples.

Tested Reactivity	rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg3230 Product name: Recombinant Rat B7-H4 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 29-257 aa of NM_001024244.1 Sequence: FGISGKHFITVTTFTSAGNIGEDGTLSCTFEPDIKLNGIVIQWLKEGIKGLVHEFKEGKDDLSQQHEMFRGRTAVFADQVVVGNASLRLKNVQLTDAGTYTCYIHTSKGKGNANLEYKTGAFSMPEINVDYNASSESLRCEAPRWFPQPTVAWASQVDQGANFSEVSNTSFELNSENVTMKVVSVLYNVTINNTYSCMIENDIAKATGDIKVTDSEVKRRSQLELLNSG Predict reactive species
Full Name	V-set domain containing T cell activation inhibitor 1
Calculated Molecular Weight	31 kDa
GenBank Accession Number	NM_001024244.1
Gene Symbol	Vtcn1
Gene ID (NCBI)	295322
RRID	AB_3746172
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q501W4
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

B7-H4, also named VTCN1, B7X, or B7S1, is a single-pass type I membrane protein, which contains two immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7-H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7-H4 is over-expressed in many cancers, including breast, ovarian, endometrial, renal cell and non-small-cell lung cancers.